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KMID : 1234420090370010010
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Korean Journal of Microbiololgy and Biotechnology
2009 Volume.37 No. 1 p.10 ~ p.16
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Isolation of Myxobacteria Carrying Soraphen Biosynthetic Gene Clusters
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Lee Cha-Yul
Hyun Hye-Sook
Cho Kyung-Yun
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Abstract
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Polymerase chain reactin (PCR) could be a simple way to screen new microbial strains producing useful secondary metabolites if their biosynthetic genes are known and candidate strains to be screened are available. In this study, we have screened two myxobacterial strains, KYC3047 and KYC3076, carrying genes appeared to be biosynthetic genes of soraphen A, a potent antifungal substance, out of 50 cellulose degrading myxobacteria using PCR. The two strains were identified as Sorangium cellulosum based on morphological, physiological, and molecular biological characteristics. Both of the strains produced substances having strong antifungal activities as expected against Candida albicans, a causative agent of candidiasis, and Colletotrichum acutatum, a causative agent of anthracnose on pepper.
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KEYWORD
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Myxobacteria, Sorangium cellulosum, soraphen
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